Comparison of methods for the extraction of DNA from stream epilithic biofilms

被引：0
|
作者：
Gavin Lear
Yimin Dong
Gillian Lewis
机构：
[1] Lincoln University,Department of Soil and Physical Sciences
[2] The University of Auckland,School of Biological Sciences
来源：
Antonie van Leeuwenhoek | 2010年 / 98卷
关键词：
Bacterial; DNA extraction kit; ARISA;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study compares how different DNA extraction methods influence the quantity and quality of DNA yields from stream epilithic biofilms. Interpretations of bacterial community structure, using ARISA, revealed increased variability among samples processed using commercially-available kits, which also yielded lower DNA concentrations than other methods tested.
引用
收藏
页码：567 / 571
页数：4
相关论文
共 50 条
  • [1] Comparison of methods for the extraction of DNA from stream epilithic biofilms
    Lear, Gavin
    Dong, Yimin
    Lewis, Gillian
    ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2010, 98 (04): : 567 - 571
  • [2] COMPARISON OF METHODS OF DNA EXTRACTION FROM STREAM SEDIMENTS
    LEFF, LG
    DANA, JR
    MCARTHUR, JV
    SHIMKETS, LJ
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (03) : 1141 - 1143
  • [3] Evaluation of DNA extraction methods from complex phototrophic biofilms
    Ferrera, Isabel
    Massana, Ramon
    Balague, Vanessa
    Pedros-Alio, Carles
    Sanchez, Olga
    Mas, Jordi
    BIOFOULING, 2010, 26 (03) : 349 - 357
  • [4] Spatial and temporal heterogeneity of the bacterial communities in stream epilithic biofilms
    Lear, Gavin
    Anderson, Marti J.
    Smith, Joanna P.
    Boxen, Kristine
    Lewis, Gillian D.
    FEMS MICROBIOLOGY ECOLOGY, 2008, 65 (03) : 463 - 473
  • [5] DNA extraction from bones -: Comparison of methods
    Müller, A
    Hummel, S
    Herrmann, B
    HOMO, 2000, 51 : S89 - S89
  • [6] Seasonal dynamics of bacterial assemblages in epilithic biofilms in a northeastern Ohio stream
    Olapade, OA
    Leff, LG
    JOURNAL OF THE NORTH AMERICAN BENTHOLOGICAL SOCIETY, 2004, 23 (04): : 686 - 700
  • [7] Evaluation of Methods for the Extraction of DNA from Drinking Water Distribution System Biofilms
    Hwang, Chiachi
    Ling, Fangqiong
    Andersen, Gary L.
    LeChevallier, Mark W.
    Liu, Wen-Tso
    MICROBES AND ENVIRONMENTS, 2012, 27 (01) : 9 - 18
  • [8] Comparison of extraction methods for quantifying extracellular polymers in biofilms
    Zhang, XQ
    Bishop, PL
    Kinkle, BK
    WATER SCIENCE AND TECHNOLOGY, 1999, 39 (07) : 211 - 218
  • [9] Methods for DNA extraction from various soils: a comparison
    Tien, CC
    Chao, CC
    Chao, WL
    JOURNAL OF APPLIED MICROBIOLOGY, 1999, 86 (06) : 937 - 943
  • [10] Comparison of four methods of DNA extraction from rice
    SONG Ming GUO Zhongxin GAO Zhiyong ZHANG Yunsun
    ChineseRiceResearchNewsletter, 2002, (Z1) : 10 - 11
12345