Structural Features of the Nucleolus in the Mouse Germinal Vesicle Oocyte Revealed by AFM, SEM, and ToF-SIMS

被引：3

作者：

Astafiev A.A. ^{[1
]}

Gulin A.A. ^{[1
,2
]}

Osychenko A.A. ^{[1
]}

Solodina A.E. ^{[1
]}

Syrchina M.S. ^{[1
]}

Titov A.A. ^{[1
]}

Shakhov A.M. ^{[1
]}

Pogorelov A.G. ^{[3
]}

Pogorelova V.N. ^{[3
]}

Panait A.I. ^{[3
]}

Nadtochenko V.A. ^{[1
,2
,4
]}

机构：

[1] Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow

[2] Department of Chemistry, Moscow State University, Moscow

[3] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast

[4] Institute of Problems of Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow oblast

来源：

Nanotechnologies in Russia | 2017年 / 12卷 / 7-8期

关键词：

D O I：

10.1134/S1995078017040048

中图分类号：

学科分类号：

摘要：

The structural organization of the biological material in the nucleoli (Nucleolus-Like Bodies (NLBs)) in mouse GV oocytes has been studied with atomic force microscopy (AFM), scanning electron microscopy (SEM), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Experimental samples are prepared by quick plunge freezing, followed by freeze-drying, embedding in Epon epoxy resin, and sectioning with a microtome. The biological material in the NLBs is found to be organized as a domain structure of densely packed granules with sizes from tenths of a micron to a micron. © 2017, Pleiades Publishing, Ltd.

引用

页码：444 / 447

页数：3

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