Construction of expression vector of CryIA(c) gene and its transformation in sugarcane

被引：6
|
作者：
Xu J.-S. [1 ]
Gao S. [1 ]
Xu L. [1 ]
Chen R. [1 ]
机构：
[1] Key Lab. of Eco-physiology and Genetics Improvement for Sugarcane, Ministry of Agriculture, Sugarcane Institute of Fujian Agriculture and Forestry University, Fuzhou
来源：
Sugar Tech | 2008年 / 10卷 / 3期
关键词：
cryIA(c) gene; Genetic transformation; Sugarcane; Sugarcane borer;
D O I：
10.1007/s12355-008-0048-3
中图分类号：
学科分类号：
摘要：
A plant expression vector, pGCryIA(c), was constructed by linking CryIA(c) gene which has UBI as promoter and NOS as terminator into pGreenII 0229. The size of pGCryIA(c) is 8,602 bp with bar (phosphinothricin acetyl transferase) gene as screening marker gene by using herbicide phosphinothricin (PPT) or Basta selection. The embryonic callus of GT94-119 was transformed with the plasmid of pUBCG0229 by bombardment. 203 regenerated plants were obtained after screening with PPT or Basta. Two transformation events were proved sucessful by PCR, PCR products sequencing and Dot-Southern blotting. © 2008 Society for Sugar Research & Promotion.
引用
收藏
页码：269 / 273
页数：4
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