Backbone and methyl resonance assignments of the 42 kDa human Hsc70 nucleotide binding domain in the ADP state

被引：0

作者：

Erik R. P. Zuiderweg

Jason E. Gestwicki

机构：

[1] The University of Michigan Medical School,Department of Biological Chemistry

[2] University of California at San Francisco,Institute for Neurodegenerative Disease

来源：

Biomolecular NMR Assignments | 2017年 / 11卷

关键词：

Hsp70; Chaperones; Automatic assignments; Methyl reprotonation;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Hsc70 is the constitutively expressed mammalian heat shock 70 kDa (Hsp70) cytosolic chaperone. It plays a central role in cellular proteostasis and protein trafficking. Here, we present the backbone and methyl group assignments for the 386-residue nucleotide binding domain of the human protein. This domain controls the chaperone’s allostery, binds multiple co-chaperones and is the target of several classes of known chemical Hsp70 inhibitors. The NMR assignments are based on common triple resonance experiments with triple labeled protein, and on several 15N and 13C-resolved 3D NOE experiments with methyl-reprotonated samples. A combination of computer and manual data interpretation was used.

引用

页码：11 / 15

页数：4

共 42 条

[1] Backbone and methyl resonance assignments of the 42 kDa human Hsc70 nucleotide binding domain in the ADP state
Zuiderweg, Erik R. P.
Gestwicki, Jason E.
BIOMOLECULAR NMR ASSIGNMENTS, 2017, 11 (01) : 11 - 15
[2] Oligomerization of the 17-kDa peptide-binding domain of the molecular chaperone HSC70
Fouchaq, B
Benaroudj, N
Ebel, C
Ladjimi, MM
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 259 (1-2): : 379 - 384
[3] Steered molecular dynamics simulation of the binding of the bovine auxilin J domain to the Hsc70 nucleotide-binding domain
You-Lin Xue
Lei Zhou
Yuna Sun
Hui Li
Gary W. Jones
Youtao Song
Journal of Molecular Modeling, 2017, 23
[4] Steered molecular dynamics simulation of the binding of the bovine auxilin J domain to the Hsc70 nucleotide-binding domain
Xue, You-Lin
Zhou, Lei
Sun, Yuna
Li, Hui
Jones, Gary W.
Song, Youtao
JOURNAL OF MOLECULAR MODELING, 2017, 23 (11)
[5] Backbone NMR resonance assignments of the nucleotide binding domain of the ABC multidrug transporter LmrA from Lactococcus lactis in its ADP-bound state
Hellmich, Ute A.
Duchardt-Ferner, Elke
Glaubitz, Clemens
Woehnert, Jens
BIOMOLECULAR NMR ASSIGNMENTS, 2012, 6 (01) : 69 - 73
[6] Backbone NMR resonance assignments of the nucleotide binding domain of the ABC multidrug transporter LmrA from Lactococcus lactis in its ADP-bound state
Ute A. Hellmich
Elke Duchardt-Ferner
Clemens Glaubitz
Jens Wöhnert
Biomolecular NMR Assignments, 2012, 6 : 69 - 73
[7] Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form
Davulcu, Omar
Niu, Xiaogang
Brueschweiler-Li, Lei
Brueschweiler, Rafael
Skalicky, Jack J.
Chapman, Michael S.
BIOMOLECULAR NMR ASSIGNMENTS, 2014, 8 (02) : 335 - 338
[8] Backbone resonance assignments of the 42 kDa enzyme arginine kinase in the transition state analogue form
Omar Davulcu
Xiaogang Niu
Lei Brüschweiler-Li
Rafael Brüschweiler
Jack J. Skalicky
Michael S. Chapman
Biomolecular NMR Assignments, 2014, 8 : 335 - 338
[9] Functional interaction of the auxilin J domain with the nucleotide- and substrate-binding modules of Hsc70
Ungewickell, E
Ungewickell, H
Holstein, SEH
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (31) : 19594 - 19600
[10] Backbone resonance assignments of the human p73 DNA binding domain
Cino, Elio A.
Soares, Iaci N.
Freitas, Mnica S.
Silva, Jerson L.
BIOMOLECULAR NMR ASSIGNMENTS, 2016, 10 (01) : 49 - 51

← 1 2 3 4 5 →