Rapid and simple purification of a novel extracellular β-amylase from Bacillus sp.

被引：0

作者：

Young Min Ha

Dong Gun Lee

Jung-Hoon Yoon

Yong-Ha Park

Young Jae Kim

机构：

[1] Changwon National University,Department of Microbiology, College of Natural Sciences

[2] Chosun University,Research Center for Proteineous Materials

[3] Korea Research Institute of Bioscience and Biotechnology,undefined

[4] KIST,undefined

来源：

Biotechnology Letters | 2001年 / 23卷

关键词：

anchovy-; sp. KYJ 963; extracellular β-amylase; starch adsorption;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Bacillus sp. KYJ 963, a local isolate, produced an extracellular amylase with Mr=59 kDa. The amylase was easily purified by adsorption on soluble starch. The analyses of TLC and N-terminal amino acid sequence from the purified protein revealed that the enzyme was a novel β-amylase which could not hydrolyze maltose or α-cyclodextrin and its N-terminal amino acid sequence was A-V-N-G-Q-S-F-N-S-N-Y-K-T-Y-K-.

引用

页码：1435 / 1438

页数：3

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