Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells

被引：44
|
作者：
Ye X. [1 ]
Wang Z.-Y. [1 ,2 ]
Wu X. [1 ]
Potrykus I. [1 ]
Spangenberg G. [1 ,2 ]
机构：
[1] Institute for Plant Sciences, Swiss Fed. Institute of Technology
[2] Plant Sciences and Biotechnology, Agriculture Victoria, La Trobe University, Bundoora
来源：
Plant Cell Reports | 1997年 / 16卷 / 6期
关键词：
Forage and turf grasses; Italian ryegrass; Lolium multiflorum; Microprojectile bombardment; Transgenic plants;
D O I：
10.1007/BF01146777
中图分类号：
学科分类号：
摘要：
Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by rice Actl 5' regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimeric β-glucuronidase (gusA) gene driven by the maize Ubi I promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.
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页码：379 / 384
页数：5
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