Single cell RNA sequencing of stem cell-derived retinal ganglion cells

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作者：
Maciej Daniszewski
Anne Senabouth
Quan H. Nguyen
Duncan E. Crombie
Samuel W. Lukowski
Tejal Kulkarni
Valentin M. Sluch
Jafar S. Jabbari
Xitiz Chamling
Donald J. Zack
Alice Pébay
Joseph E. Powell
Alex W. Hewitt
机构：
[1] Centre for Eye Research Australia,Department of Surgery
[2] Royal Victorian Eye and Ear Hospital,Departments of Neuroscience
[3] Ophthalmology,undefined
[4] the University of Melbourne,undefined
[5] Institute for Molecular Bioscience,undefined
[6] University of Queensland,undefined
[7] St Lucia,undefined
[8] Wilmer Eye Institute,undefined
[9] Johns Hopkins University School of Medicine,undefined
[10] Australian Genome Research Facility,undefined
[11] Molecular Biology and Genetics,undefined
[12] and Institute of Genetic Medicine,undefined
[13] Johns Hopkins University School of Medicine,undefined
[14] Queensland Brain Institute,undefined
[15] University of Queensland,undefined
[16] St Lucia,undefined
[17] School of Medicine,undefined
[18] Menzies Institute for Medical Research,undefined
[19] University of Tasmania,undefined
来源：
Scientific Data | / 5卷
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摘要：
We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more mature RGC phenotype.
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