Identification of a Homopolymer of 5-Acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic Acid in the Lipopolysaccharides of Legionella pneumophila Non-1 Serogroups

被引：0
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作者：
Y. A. Knirel
S. N. Senchenkova
N. A. Kocharova
A. S. J. H. U. Shashkov
机构：
[1] Russian Academy of Sciences,Zelinsky Institute of Organic Chemistry
[2] Center for Medicine and Biosciences,Research Center Borstel
[3] University Clinic of the Dresden Technical University,Institute of Medical Microbiology and Hygiene
来源：
Biochemistry (Moscow) | 2001年 / 66卷
关键词：
lipopolysaccharide; O-specific polysaccharide; structure; 5-acetamidino-7-acetamido-3,5,7; 9-tetradeoxy-D-glycero-D-talo-nonulosonic acid; legionaminic acid; Legionella pneumophila;
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摘要：
O-Specific polysaccharides (OPS) were isolated by mild acid hydrolysis of the lipopolysaccharides (LPS) of strainsof Legionella pneumophila serogroups 2-14, as well as strains Lansing 3 and 16453-92 from newly proposed serogroups. TheOPS were studied by 1H- and 13C-NMR spectroscopy, GLC/mass spectrometry, and chemical modifications (mild alkalineO-deacetylation and conversion of the N-acetimidoyl group into the N-acetyl group). All OPS were found to be ahomopolymer of a 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxynonulosonic acid, which in some strains is 8-O-acetylat-ed. In most strains studied, the monosaccharide has the D-glycero-D-talo configuration and is thus the C4 epimer of legion-aminic acid (4-epilegionaminic acid), which has been previously identified as the monomer in the OPS of L. pneumophilaserogroup 1. Poly(4-epilegionaminic acid) occurs as a minor polysaccharide in serogroups 5 (strain Dallas 1) and 13 and isabsent in serogroups 1 and 7. The chemical basis for serological differentiation of L. pneumophila strains is discussed.
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页码：1035 / 1041
页数：6
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