The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria

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作者
Timothy D. Read
Scott N. Peterson
Nicolas Tourasse
Les W. Baillie
Ian T. Paulsen
Karen E. Nelson
Hervé Tettelin
Derrick E. Fouts
Jonathan A. Eisen
Steven R. Gill
Erik K. Holtzapple
Ole Andreas Økstad
Erlendur Helgason
Jennifer Rilstone
Martin Wu
James F. Kolonay
Maureen J. Beanan
Robert J. Dodson
Lauren M. Brinkac
Michelle Gwinn
Robert T. DeBoy
Ramana Madpu
Sean C. Daugherty
A. Scott Durkin
Daniel H. Haft
William C. Nelson
Jeremy D. Peterson
Mihai Pop
Hoda M. Khouri
Diana Radune
Jonathan L. Benton
Yasmin Mahamoud
Lingxia Jiang
Ioana R. Hance
Janice F. Weidman
Kristi J. Berry
Roger D. Plaut
Alex M. Wolf
Kisha L. Watkins
William C. Nierman
Alyson Hazen
Robin Cline
Caroline Redmond
Joanne E. Thwaite
Owen White
Steven L. Salzberg
Brendan Thomason
Arthur M. Friedlander
Theresa M. Koehler
Philip C. Hanna
机构
[1] The Institute for Genomic Research,Medical Biotechnology Center
[2] University of Maryland Biotechnology Institute,Department of Biochemistry
[3] The George Washington University,Department of Microbiology and Tropical Medicine
[4] The George Washington University,Department of Pharmacology
[5] The George Washington University,School of Pharmacy
[6] University of Oslo,Department of Microbiology & Immunology
[7] Defence Science Technology Laboratory,Department of Microbiology and Molecular Genetics
[8] Johns Hopkins University,undefined
[9] The Biotechnology Center of Oslo,undefined
[10] University of Michigan Medical School,undefined
[11] Immunology,undefined
[12] University of Michigan Medical School,undefined
[13] US Army Medical Research Institute for Infectious Diseases,undefined
[14] University of Texas–Houston Health Science Center Medical School,undefined
[15] University of Texas,undefined
来源
Nature | 2003年 / 423卷
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摘要
Bacillus anthracis is an endospore-forming bacterium that causes inhalational anthrax1. Key virulence genes are found on plasmids (extra-chromosomal, circular, double-stranded DNA molecules) pXO1 (ref. 2) and pXO2 (ref. 3). To identify additional genes that might contribute to virulence, we analysed the complete sequence of the chromosome of B. anthracis Ames (about 5.23 megabases). We found several chromosomally encoded proteins that may contribute to pathogenicity—including haemolysins, phospholipases and iron acquisition functions—and identified numerous surface proteins that might be important targets for vaccines and drugs. Almost all these putative chromosomal virulence and surface proteins have homologues in Bacillus cereus, highlighting the similarity of B. anthracis to near-neighbours that are not associated with anthrax4. By performing a comparative genome hybridization of 19 B. cereus and Bacillus thuringiensis strains against a B. anthracis DNA microarray, we confirmed the general similarity of chromosomal genes among this group of close relatives. However, we found that the gene sequences of pXO1 and pXO2 were more variable between strains, suggesting plasmid mobility in the group. The complete sequence of B. anthracis is a step towards a better understanding of anthrax pathogenesis.
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页码:81 / 86
页数:5
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