Analysis of the effects of mesoporous silica particles SBA-15 and SBA-16 in Streptococcus pneumoniae transformation process

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作者
Maria-Cecília Krähenbühl Amstalden
José Diogo Oliveira
Mathias Strauss
Italo Odone Mazali
Daisy Machado
Thais Holtz Theisen
Marcelo Lancellotti
机构
[1] University of Campinas,Department of Biochemistry and Tissue Biology, Institute of Biology
[2] University of Campinas,Faculty of Pharmaceutical Sciences
[3] Brazilian Nanotechnology National Laboratory,Functional Materials Laboratory, Institute of Chemistry
[4] National Center for Research in Energy and Materials,undefined
[5] University of Campinas,undefined
来源
Folia Microbiologica | 2019年 / 64卷
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摘要
Streptococcus pneumoniae are natural competent bacteria which requires the presence of a pheromone-like molecule to do the transformation process. This study verified the influence of mesoporous silica (SBA-15 and SBA-16) on the transformation process in S. pneumoniae using a donor DNA obtained from a mutant strain of this microorganism (Sp360∆luxS). The results showed that mesoporous silica SBA-15 and SBA-16 particles doubled the transformation ratio frequency compared with negative control (without nanoparticles) in using SBA-15 (ratio 1.81 ± 0.04) and SBA-16 (ratio 2.18 ± 0.22). We demonstrated the how mesoporous silica nanoparticles were able to increase the pneumococcus transformations, which could possibly lead to the acquisition of virulence factor genes and resistance of antibiotics.
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页码:127 / 132
页数:5
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