In this article, we investigate harmonicity, Laplacians, mean value theorems, and related topics in the context of quaternionic analysis. We observe that a Mean Value Formula for slice regular functions holds true and it is a consequence of the well-known Representation Formula for slice regular functions over H\documentclass[12pt]{minimal}
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\begin{document}$${\mathbb {H}}$$\end{document}. Motivated by this observation, we have constructed three order-two differential operators in the kernel of which slice regular functions are, answering positively to the question: is a slice regular function over H\documentclass[12pt]{minimal}
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\begin{document}$${\mathbb {H}}$$\end{document} (analogous to an holomorphic function over C\documentclass[12pt]{minimal}
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\begin{document}$${\mathbb {C}}$$\end{document}) ”harmonic” in some sense, i.e., is it in the kernel of some order-two differential operator over H\documentclass[12pt]{minimal}
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\begin{document}$${\mathbb {H}}$$\end{document}? Finally, some applications are deduced such as a Poisson Formula for slice regular functions over H\documentclass[12pt]{minimal}
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\begin{document}$${\mathbb {H}}$$\end{document} and a Jensen’s Formula for semi-regular ones.