Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors

被引：0
|
作者：
Nikos Vasilakis
Darlene Falvey
Seema S Gangolli
John Coleman
Jacek Kowalski
Stephen A Udem
Timothy J Zamb
Gerald R Kovacs
机构：
[1] Viral Vaccine Discovery,Department of Pathology
[2] Wyeth Vaccines Research,undefined
[3] University of Texas Medical Branch at Galveston,undefined
[4] Office of Biodefense Research Affairs,undefined
[5] National Institute of Allergy and Infectious Diseases,undefined
[6] National Institutes of Heath,undefined
来源：
Nature Biotechnology | 2003年 / 21卷
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摘要：
This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use.
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页码：932 / 935
页数：3
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