Isolation and characterization of nuclear microsatellite primers for the Barbary thuja, Tetraclinis articulata (Vahl) Mast. (Cupressaceae)

被引：0
|
作者：
María Teresa Lorenzo
Ramón Casimiro-Soriguer
Francisco Balao
Juan Luis García-Castaño
Jose M. Sánchez-Robles
Anass Terrab
机构：
[1] Universidad de Sevilla,Departamento de Biología Vegetal y Ecología
[2] University of Vienna,Department of Systematic and Evolutionary Botany
[3] Universidad de Cádiz,Departamento de Biología
来源：
Conservation Genetics Resources | 2014年 / 6卷
关键词：
Cupressaceae; Microsatellites; Population genetics; nSSR;
D O I：
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中图分类号：
学科分类号：
摘要：
Fifty nuclear microsatellite primers were initially developed using next-generation sequencing (454) data from a single individual of Tetraclinis articulata (Vahl) Mast. Eleven primers were finally applied in 30 individuals from 3 localities from Morocco, Algeria and Spain. The number of alleles per locus ranged from one to ten. The average observed and expected heterozygosities across the populations studied ranged from 0.10 to 0.80 and from 0.09 to 0.88, respectively. The microsatellite markers described here are valuable tools for the population genetic research of T. articulata, and can be used to obtain information for creating suitable management strategies to conserve this endemic and endangered species.
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页码：233 / 235
页数：2
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