Enhanced enzymatic hydrolysis of mild alkali pre-treated rice straw at high-solid loadings using in-house cellulases in a bench scale system

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作者
Madhuri Narra
Velmurugan Balasubramanian
Jisha P. James
机构
[1] Sardar Patel Renewable Energy Research Institute,
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关键词
In-house cellulases; Mild alkali pretreatment; High-solid saccharification; Concentrated fermentable sugars;
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摘要
In the present study, scale-up systems for cellulase production and enzymatic hydrolysis of pre-treated rice straw at high-solid loadings were designed, fabricated and tested in the laboratory. Cellulase production was carried out using tray fermentation at 45 °C by Aspergillus terreus in a temperature-controlled humidity chamber. Enzymatic hydrolysis studies were performed in a horizontal rotary drum reactor at 50 °C with 25 % (w/v) solid loading and 9 FPU g−1 substrate enzyme load using in-house as well commercial cellulases. Highly concentrated fermentable sugars up to 20 % were obtained at 40 h with an increased saccharification efficiency of 76 % compared to laboratory findings (69.2 %). These findings demonstrate that we developed a simple and less energy intensive bench scale system for efficient high-solid saccharification. External supplementation of commercial β-glucosidase and hemicellulase ensured better hydrolysis and further increased the saccharification efficiency by 14.5 and 20 %, respectively. An attempt was also made to recover cellulolytic enzymes using ultrafiltration module and nearly 79–84 % of the cellulases and more than 90 % of the sugars were recovered from the saccharification mixture.
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页码:993 / 1003
页数:10
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    [J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2016, 39 (06) : 993 - 1003
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