The dried corncob as a source of DNA for PCR analysis

被引:0
|
作者
M. C. Schneerman
J. Mwangi
B. Hobart
J. Arbuckle
D. A. Vaske
J. C. Register
D. F. Weber
机构
[1] University of Illinois,Department of Crop Sciences
[2] Illinois State University,undefined
[3] Pioneer Hi-Bred International,undefined
[4] Inc.,undefined
[5] Sygenta,undefined
来源
Plant Molecular Biology Reporter | 2002年 / 20卷
关键词
corncob; DNA; PCR;
D O I
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中图分类号
学科分类号
摘要
We developed a procedure to isolate DNA from dried corncobs. This DNA was amplified successfully by PCR, producing well-defined bands in response to specific primers. The SSR patterns between cob and leaf DNA of the same inbred line were found to be identical, indicating that the DNA in the cob cells had not degraded during senescence.
引用
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页码:59 / 65
页数:6
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