Hymenobacter lucidus sp. nov., and Hymenobacter nitidus sp. nov., isolated from soil

Two novel bacterial strains BT178T and BT635T were isolated from soil collected in Gyeonggi province, Republic of Korea. Both strains were Gram-negative, rod-shaped circular, and had convex, glistening, pink-colored colonies. Phylogenetic analysis based on 16S rRNA gene sequences showed that each of the strains BT178T and BT635T formed an individual lineage within the genus Hymenobacter (family Hymenobacteraceae, order Cytophagales, class Cytophagia, phylum Bacteroidetes). The 16S rRNA gene sequence similarity between the two strains BT178T and BT635T were 97.7%. Both strains had Q-7 as the major respiratory quinone and phosphatidylethanolamine (PE) as the major polar lipids. The main cellular fatty acids of strain BT178T were iso-C15:0, anteiso-C15:0, and those of strain BT635T were iso-C15:0 and Summed Feature 4 (iso-C17:1 I / anteiso-C17:1 B). Based on the result of polyphasic taxonomic analysis, two novel species, Hymenobacter lucidus BT178T (= KCTC 72333T = NBRC 115442T) and Hymenobacter nitidus BT635T (= KACC 21904T = NBRC 115443T) were proposed.