Biosynthesis of silver nanoparticles using mushroom extract and its toxicity assessement in zebrafish embryos

The study undertakes the synthesis of “Silver Nanoparticles” (AgNPs) via biological approach in order to overcome the toxic effects caused by synthesis using conventional chemical methods by conducting in vivo toxicity in zebrafish embryos. Edible oyster mushroom P. ostreatus were utilized for synthesizing AgNPs in aqueous phase. 1 mM silver nitrate when combined with the prepared aqueous extract of P. ostreatus, causes reduction of silver nitrate to form nanoparticles of silver which were characterized using UV–VIS Spectroscopy, Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red study (FTIR) techniques. In vivo toxicity assessment was carried out as per OECD guidelines for Fish Embryo Toxicity (FET). Study showed dose-dependent toxicity to different concentrations of biosynthesized AgNPs on exposure to zebrafish embryos and LC50 value was determined to be 1.8 mg/L. © 2022, The Author(s) under exclusive licence to Society for Plant Research.