Controlled gene expression in primary Lgr5 organoid cultures

被引：0
|
作者：
Koo B.-K. [1 ]
Stange D.E. [1 ]
Sato T. [1 ,2 ]
Karthaus W. [1 ]
Farin H.F. [1 ]
Huch M. [1 ]
Van Es J.H. [1 ]
Clevers H. [1 ]
机构：
[1] Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre, Utrecht
[2] Department of Gastroenterology, School of Medicine, Keio University, Tokyo
来源：
Nature Methods | 2012年 / 9卷 / 1期
基金：
欧洲研究理事会; 新加坡国家研究基金会;
关键词：
D O I：
10.1038/nmeth.1802
中图分类号：
学科分类号：
摘要：
The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinaseinducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems. © 2012 Nature America, Inc. All rights reserved.
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页码：81 / 83
页数：2
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