High-throughput generation of selected reaction-monitoring assays for proteins and proteomes

被引：0

作者：

Picotti P. ^{[1
]}

Rinner O. ^{[1
,2
]}

Stallmach R. ^{[1
]}

Dautel F. ^{[3
]}

Farrah T. ^{[4
]}

Domon B. ^{[1
]}

Wenschuh H. ^{[5
]}

Aebersold R. ^{[1
,4
,6
,7
]}

机构：

[1] Institute of Molecular Systems Biology, Swiss Federal Institute of Technology, Zurich

[2] Biognosys AG, Zurich

[3] Department of Proteomics, Helmholtz Centre for Environmental Research, Leipzig

[4] Institute for Systems Biology, Seattle, WA

[5] JPT Peptide Technologies GmbH, Berlin

[6] Competence Center for Systems Physiology and Metabolic Diseases, Zurich

[7] Faculty of Science, University of Zurich, Zurich

来源：

Nature Methods | 2010年 / 7卷 / 1期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.1408

中图分类号：

学科分类号：

摘要：

Selected reaction monitoring (SRM) uses sensitive and specific mass spectrometric assays to measure target analytes across multiple samples, but it has not been broadly applied in proteomics owing to the tedious assay development process for each protein. We describe a method based on crude synthetic peptide libraries for the high-throughput development of SRM assays. We illustrate the power of the approach by generating and applying validated SRM assays for all Saccharomyces cerevisiae kinases and phosphatases. © 2010 Nature America, Inc. All rights reserved.

引用

页码：43 / 46

页数：3

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