Sulfate assimilation in Aspergillus terreus: analysis of genes encoding ATP-sulfurylase and PAPS-reductase

被引:0
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作者
M. Schierová
M. Linka
S. Pažoutová
机构
[1] Faculty of Sciences,
[2] Charles University,undefined
[3] Viniěná 5,undefined
[4] 12800 Prague 2,undefined
[5] Czech Republic,undefined
[6] Institute of Microbiology,undefined
[7] Academy of Sciences of the Czech Republic,undefined
[8] Vídeňnská 1083,undefined
[9] 14220 Prague 4,undefined
[10] Czech Republic e-mail: pazouto@biomed.cas.cz,undefined
来源
Current Genetics | 2000年 / 38卷
关键词
Key wordsAspergillus terreus; Sulfur assimilation; ATP-Sulfurylase; PAPS-Reductase;
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摘要
Two genes for the sulfate assimilation pathway in Aspergillus terreus were cloned. The genes sAT (coding for PAPS-reductase) and sCT (coding for ATP-sulfurylase) form a small gene cluster. Both genes are similar to their homologs in A. nidulans (sA and sC), Penicillium chrysogenum (aps) and Saccharomyces cerevisiae (MET3 and MET16). In the coding sequence of the sCT gene, a typical non-functional APS-kinase-like domain is present. The sCT gene is expressed in A. nidulans, but its expression there is less sensitive to methionine level than in the original species. Two regions 5′ upstream of sAT were found to be similar to those of sA.
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页码:126 / 131
页数:5
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