Neuro-fuzzy prediction of uricase production

Recent biotechnology requires implementation of new modelling methods based on knowledge principles and learning structures, comprised in fuzzy knowledge-based systems (FKBS), neural networks (NN) and different hybrid methods. The intelligent modelling approaches solve sufficiently a very important problem – processing of scarce, uncertainty and incomplete numerical and linguistic information about multivariate non-linear and non-stationary systems as well as biotechnological processes. The paper deals with prediction of an enzyme oxidizing uric acid to alantoin – the uricase, produced by Candida utilis 90-12 employing neuro-fuzzy knowledge-based approach. The implemented predictive technique exploits the fact that the fuzzy model can be seen as a network structure, similar to artificial NN, which on computational level assure a high model accuracy. The predictors implemented are four different by nature variables. The developed predictive model shows that best predictors of uricase production are biomass and limiting substrate concentrations.