Combined Application of Biochar and Biocontrol Agents Enhances Plant Growth and Activates Resistance Against Meloidogyne incognita in Tomato; [Die kombinierte Anwendung von Biokohle und biologischen Schädlingsbekämpfungsmitteln verbessert das Pflanzenwachstum und aktiviert die Resistenz gegen Meloidogyne incognita bei Tomaten]

被引：0

作者：

Arshad U. ^{[1
,9
]}

Azeem F. ^{[2
]}

Mustafa G. ^{[3
]}

Bakhsh A. ^{[4
]}

Toktay H. ^{[5
]}

McGiffen M. ^{[6
]}

Nawaz M.A. ^{[7
]}

Naveed M. ^{[8
]}

Ali M.A. ^{[3
,9
]}

机构：

[1] Department of Plant Pathology, The Islamia University of Bahawalpur, Bahawalpur

[2] Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad

[3] Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Faisalabad

[4] Centre of Excellence in Molecular Biology (CEMB), University of Punjab, Lahore

[5] Department of Plant Production and Technologies, Nigde Omer Halisdemir University, Nigde

[6] Department of Botany and Plant Sciences, University of California Riverside, Riverside

[7] Siberian Federal Scientific Centre of Agrobiotechnology, Russian Academy of Sciences, Krasnoobsk, Novosibirsk region

[8] Institute of Soil and Environmental Science, University of Agriculture, Faisalabad

[9] Department of Plant Pathology, University of Agriculture, Faisalabad

来源：

Gesunde Pflanzen | 2021年 / 73卷 / 4期

关键词：

Biochar; Biocontrol; Defense-related genes; Gene expression; Meloidogyne incognita;

D O I：

10.1007/s10343-021-00580-4

中图分类号：

学科分类号：

摘要：

Biochar (BC) is known to enhance plant growth and may activate plant resistance to various soil borne pathogens. In this experiment, the rice husk (RH) BC was combined with biocontrol agents (BCAs), Bacillus subtilis and Trichoderma harzianum against Meloidogyne incognita in tomato and the efficacy of BC and BCAs in boosting overall plant biomass assessed side by side. BCAs along with 3% RH BC were applied to potting soil, tomato plants were transplanted, and then the soil was inoculated with second stage juveniles of Meloidogyne incognita. Bacillus subtilis +3% RH BC improved overall plant growth and reduced M. incognita damage. To unravel the resistance at the molecular level, root samples were taken 6‑ and 12-days post inoculation (dpi) to measure the expression of two resistance genes, PR-1b and JERF3, with the help of quantitative real-time polymerase chain reaction (qRT-PCR). The expression of PR-1b was upregulated 6 dpi for only BCAs while JERF3 remained unchanged except in only Bacillus subtilis treated plants. Similarly, the expression of both genes was found to be upregulated in some combined treatments at 12 dpi. The results suggested that the 3% BC application with BCAs effectively manages root-knot nematode, enhances overall plant biomass, and triggers defense related genes in tomato plants. © 2021, Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature.

引用

页码：591 / 601

页数：10

共 1 条

[1] Combined Application of Biochar and Biocontrol Agents Enhances Plant Growth and Activates Resistance Against Meloidogyne incognita in Tomato
Arshad, Usman
Azeem, Farrukh
Mustafa, Ghulam
Bakhsh, Allah
Toktay, Halil
McGiffen, Milt
Nawaz, Muhammad Amjad
Naveed, Muhammad
Ali, Muhammad Amjad
[J]. GESUNDE PFLANZEN, 2021, 73 (04): : 591 - 601

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