Spatial Diversity in Aspergillus niger and Yeast Cultures in Terms of Starch Saccharification and Bioethanol Production

In this study, soil and corn samples of distinct areas were used to isolate strains of A. niger and yeast and variable colony counts per area were studied for starch saccharification and ethanol fermentation. Highest (57%) starch saccharification was from the A. niger isolated from the soil sample of Rabi, whereas Mardan Motorway Interchange was less productive in starch saccharification. The corn sample of Takhtbhai was highest (55%) in starch saccharification, in comparison to Malakhandher. Similarly, yeast strains from the soil samples of Jhandu and corn samples of Jalala produced higher ethanol distillate (29 and 19 mL) compared to the yeast cultures isolated from other areas. Corn and soil samples of Malakandher and Jalala attained highest bioethanol recovery per 100 mL of distillate, respectively. Distinct strains of A. niger and yeast present in different areas showed great diversity in saccharification and fermentation, which might be due to variations in microbial strains and the environment around them. The best of A. niger and yeast strains could be used in bioethanol and other industries as a raw material.