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Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
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|作者:
Sunil P. Gandhi
Charles F. Stevens
机构:
[1] University of California,Neurosciences Program
[2] Howard Hughes Medical Institute,undefined
[3] and Molecular Neurobiology Laboratory,undefined
[4] The Salk Institute,undefined
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摘要:
Synapses recycle their spent vesicles in order to keep up with on-going neurotransmitter release. To investigate vesicle recycling in the small synapses of hippocampal neurons, we have used an optical recording method that permits us to resolve single-vesicle events. Here we show that an exocytic event can terminate with three modes of vesicle retrieval: a fast (400–860 ms) ‘kiss-and-run’ mode that has a selective fusion pore; a slow (8–21 s) ‘compensatory’ mode; and a ‘stranded’ mode of recycling, in which a vesicle is left on the cell surface until a nerve impulse triggers its retrieval. We have also observed that, in response to a nerve impulse, synapses with low release probability primarily use the kiss-and-run mode, whereas high release probability terminals predominantly use the compensatory mode of vesicle retrieval.
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页码:607 / 613
页数:6
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