The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells

被引:0
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作者
Sarah X L Huang
Michael D Green
Ana Toste de Carvalho
Melanie Mumau
Ya-Wen Chen
Sunita L D'Souza
Hans-Willem Snoeck
机构
[1] Columbia Center for Translational Immunology,Department of Medicine
[2] Columbia University Medical Center,Department of Developmental and Regenerative Biology
[3] Columbia University Medical Center,Department of Microbiology and Immunology
[4] Black Family Stem Cell Institute,undefined
[5] Experimental Therapeutic Institute,undefined
[6] Icahn School of Medicine at Mount Sinai,undefined
[7] Columbia University Medical Center,undefined
来源
Nature Protocols | 2015年 / 10卷
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摘要
This protocol describes how to direct differentiation of human pluripotent stem cells into developmental lung progenitors, and subsequently into predominantly distal lung epithelial cells, by following four stages that recapitulate lung development.
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页码:413 / 425
页数:12
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