First molecular characterization and phylogenetic analysis of the VP2 gene of feline panleukopenia virus in Bangladesh

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作者
Q M Monzur Kader Chowdhury
Shahrul Alam
Md. Shahidur Rahman Chowdhury
Mahmudul Hasan
Md Bashir Uddin
Md. Mukter Hossain
Md. Rafiqul Islam
Md. Masudur Rahman
Md. Mahfujur Rahman
机构
[1] Sylhet Agricultural University,Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences
[2] Sylhet Agricultural University,Department of Pharmaceuticals and Industrial Biotechnology, Faculty of Biotechnology and Genetic Engineering
[3] Sylhet Agricultural University,Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences
[4] Mississippi State University,Department of Comparative Biomedical Sciences, College of Veterinary Medicine
[5] Christian Albrechts University of Kiel,Faculty of Agricultural and Nutritional Sciences
来源
Archives of Virology | 2021年 / 166卷
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摘要
Feline panleukopenia virus (FPV) is a highly contagious infectious pathogen of cats globally. However, there is no information on the molecular identification and characterization of FPV in Bangladesh. Here, 8.16% (8/98) and 18.37% (18/98) of diarrheic cats tested positive for FPV by an immunochromatography (IC) test and PCR, respectively. The IC test showed 44.44% sensitivity and 100% specificity in comparison with PCR. Our newly sequenced Bangladeshi FPV strain (MN826076) showed the highest (99.71%) sequence identity to strains from the United Arab Emirates (UAE). Strain MN826076 contained two characteristic amino acid variations in VP2 identifying it as an FPV strain: valine at position 103 and aspartic acid at position 323. Phylogenetically, the VP2 of strain MN826076 was found to be closely related to 19 FPV strains, sharing the same clade.
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页码:2273 / 2278
页数:5
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