Expression of cellulase genes in Saccharomyces cerevisiae via δ-integration subject to auxotrophic markers

被引：0
|
作者：
Lin Liu
Cheng Liu
Shaolan Zou
Huajun Yang
Jiefang Hong
Yuanyuan Ma
Minhua Zhang
机构：
[1] Tianjin University,Tianjin R&D Center for Petrochemical Technology
[2] Tianjin University,School of Chemical Engineering and Technology
来源：
Biotechnology Letters | 2013年 / 35卷
关键词：
Auxotrophic marker; Endoglucanase; -Glucosidase; -Integration;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase) were integrated into the S. cerevisiae W303-1A chromosome as reporters, by δ-integration with the aforementioned auxotrophic markers. Eight strains, L-BGL, H-BGL, T-BGL, U-BGL, L-EG, H-EG, T-EG, and U-EG, were selected and tested. After 5 days growth, cellulase activities (U ml−1) were 3, 2.3, 1.8, 1.5, 29, 12, 8, and 1.5, respectively. The results showed that auxotrophic markers influenced the expression of cellulase genes.
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页码：1303 / 1307
页数：4
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