Metrological support for DNA sequencing

被引：0
|
作者：
S. S. Golubev
S. A. Kononogov
Yu. A. Kudeyarov
A. V. Mardanov
P. Yu. Nikolaeva
N. V. Ravin
K. G. Skryabin
机构：
[1] All-Russia Research Institute of Metrological Service (VNIIMS),
[2] Bioengineering Center,undefined
[3] Russian Academy of Sciences,undefined
来源：
Measurement Techniques | 2012年 / 55卷
关键词：
DNA molecule; nucleotide sequence; complementarity; pyrophosphate sequencing (pyrosequencing); reference material; plasmid pUC18;
D O I：
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中图分类号：
学科分类号：
摘要：
We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.
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页码：345 / 350
页数：5
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