Somatic embryogenesis of Gentiana genus III. Characterization of three-year-old embryogenic suspensions of G. pannonica originated from various seedling explants

被引：0

作者：

Anna Mikuła

Janusz Skierski

Jan J. Rybczyński

机构：

[1] Botanical Garden - Centre for Biological Diversity Conservation of the Polish Academy of Sciences,

[2] Drug Institute,undefined

[3] Flow Cytometry Laboratory,undefined

来源：

Acta Physiologiae Plantarum | 2002年 / 24卷

关键词：

seedling explants; cell suspension; long term culture; somatic embryogenesis; germlings; flow cytometry;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Experiments were carried out with three-year-old embryogenic suspension culture of Gentiana pannonica Scop. The initial explant for the suspension determinated both the embryogenic character and embryo production. Cultures were initiated by culture of hypocotyl, cotyledon and root explants on MS (Murashige and Skoog 1962) medium supplemented with 1.0 mg·l−1 Kinetin and 0.5 mg·l−1 2,4-D, later transferred and maintained in liquid MS medium with 1.0 mg·l−1 Dicamba, 0.1 mg·l−1 NAA, 2.0 mg·l−1 BAP and 80.0 mg·l−1 AS. Regeneration medium included 0.0–1.0 mg·l−1 GA3+0.0−2.0 mg·l−1 Kin.+0.0−160 mg·l−1 AS.

引用

页码：311 / 322

页数：11