CdSe/ZnS core shell quantum dot-based FRET binary oligonucleotide probes for detection of nucleic acids

被引：0
|
作者：
Yiru Peng
Chunmei Qiu
Steffen Jockusch
Amy M. Scott
Zengmin Li
Nicholas J. Turro
Jingyue Ju
机构：
[1] Columbia University,Department of Chemistry
[2] Columbia University,Department of Chemical Engineering
[3] Fujian Normal University,College of Chemistry & Material Sciences
来源：
Photochemical & Photobiological Sciences | 2012年 / 11卷
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摘要：
We report the design, synthesis, and characterization of a binary oligonucleotide probe for selective DNA or RNA detection. The probe is based on fluorescence resonance energy transfer (FRET) from quantum dot (CdSe/ZnS core shell) DNA conjugates to organic dye (cyanine-5) DNA conjugates. Selective hybridization of the donor/acceptor DNA conjugates to target DNA enhances FRET and a change in fluorescence signature was observed.
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页码：881 / 884
页数：3
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