Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeast Yarrowia hpolytica

被引：0
|
作者：
Juretzek T. [1 ]
Wang H.-J. [2 ]
Nicaud J.-M. [2 ]
Mauersberger S. [1 ]
Barth G. [1 ]
机构：
[1] Institut für Mikrobiologie, Technische Universität Dresden, D-01062 Dresden
[2] Laboratoire de Microbiologie et de Genetique Moleculaire et Cellulaire, INRA Centre de Grignon, F-78850 Thiverval-Grignon
来源：
Biotechnology and Bioprocess Engineering | 2000年 / 5卷 / 5期
关键词：
Expression vectors; lacZ expression; Regulated promoters; Yarrowia lipolytica;
D O I：
10.1007/BF02942206
中图分类号：
学科分类号：
摘要：
Promoters of the genes G3P, ICL1, POT1, POX1, POX2 and POX5 of the yeast Y. lipolytica were studied in respect to their regulations and activities during growth on different carbon sources. The aim of this study was to select suitable promoters for high expression of heterologous genes in this yeast. For this purpose the promoters were fused with the reporter gene lacZ of E. coli and integrated as single copies into the genome of Y. lipolytica strain PO1d. The measurement of expressed activities of β galactosidase revealed that pICL4, pPOX2 and pPOT4 are the strongest regulable promoters available for Y. lipolytica, at present. pPOX2 and pPOT1 were highly induced during growth on oleic acid and were completely repressed by glucose and glycerol, pICL1 was strongly inducible by ethanol besides alkanes and fatty acids, however, not completely repressible by glucose or glycerol. Ricinoleic acid methyl ester appeared as a very strong inducer for pPOT1 and pPOX2, in spite of that it inhibited growth of Y. lipolytica transformants.
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页码：320 / 326
页数：6
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