Laboratory test system for the evaluation of nanomaterial toxicity on Dunaliella salina microalgae

被引：5
|
作者：
Bogatyrev V.A. [1 ,2 ]
Golubev A.A. [1 ,2 ]
Selivanov N.Y. [1 ]
Prilepskii A.Y. [1 ]
Bukina O.G. [1 ]
Pylaev T.E. [1 ]
Bibikova O.A. [3 ]
Dykman L.A. [1 ]
Khlebtsov N.G. [1 ,2 ]
机构：
[1] Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov
[2] Chernyshevsky State University, ul. Astrakhanskaya 83, Saratov
[3] Biocenter Oulu, University of Oulu, P.O. Box 5000, Oulu
来源：
Nanotechnologies in Russia | 2015年 / 10卷 / 1-2期
基金：
俄罗斯基础研究基金会;
关键词：
CTAB; Colloidal Gold; CTAB Concentration; CTAB Solution; Material Safety Data Sheet;
D O I：
10.1134/S1995078015010048
中图分类号：
学科分类号：
摘要：
We are reporting a new diagnostic test system for evaluating nanomaterial toxicity on halophile green microalgae Dunaliella salina based on colorimetric measurements enabling us to estimate cell death and growth-rate inhibition in microalgae suspension for serial dilutions of several toxicants. The principle of the method is the photodegradation of chlorophyll from damaged cells, resulting in the discoloration of the cell suspension, which enables us to estimate the microalgae population viability. The cell response was studied for two standard pollutants: formaldehyde and hydrogen peroxide. The toxic effect of free CTAB (cetyltrimetilammonium bromide, cationic detergent) water solution was compared with the effect of gold nanoparticles: nanorods, nanostars, and nanospheres, all synthesized in CTAB solutions. It was shown that toxicity is mainly caused by free (nonabsorbed on nanoparticles) surfactant molecules. The toxicity on microalgae D. salina decreases in the following order of silver-containing compounds: silver nitrate > silver proteinate ≫ colloidal silver in same order. © 2015, Pleiades Publishing, Ltd.
引用
收藏
页码：109 / 119
页数：10
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