New record on karyological analysis and first study of NOR localization of parthenogenetic brahminy blind snake, Ramphotyphlops braminus (Squamata, Typhlopidae) in Thailand

被引:7
|
作者
Patawang I. [1 ]
Tanomtong A. [1 ]
Kaewmad P. [2 ]
Chuaynkern Y. [1 ]
Duengkae P. [3 ]
机构
[1] Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Muang, 40002, Khon Kaen
[2] Major of Biology, Faculty of Science and Technology, Mahasarakham Rajabhat University, Muang, 44000, Mahasarakham
[3] Department of Forest Biology, Faculty of Forestry, Kasetsart University, Chatuchak, 10900, Bangkok
关键词
Chromosome; Karyotype; Ramphotyphlops braminus;
D O I
10.1007/s13237-015-0154-z
中图分类号
学科分类号
摘要
This is chromosomal analysis of the brahminy blind snake, Ramphotyphlops braminus (Daudin, 1803), a newly recorded species in northeastern Thailand. Mitotic chromosomes were prepared directly from intestinal epithelia cells of five brahminy blind snakes. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the triploid chromosome number was 3n = 42, while the fundamental number (NF) was 78. The types of chromosomes were 24 macrochromosomes (19 m + 5sm) and 18 microchromosomes (9 m + 3sm + 6 t). The region adjacent to the short arm near the telomere of one in macrochromosome pair 8 showed a clearly observable nucleolar organizer region/NOR. There were no irregularly sized chromosomes related to sex. The karyotype formula for R. braminus is as follows: 3n (42) = Lm 12+ Sm 7+ Ssm 5+ 18 microchromosomes. © 2016, Archana Sharma Foundation of Calcutta.
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页码:61 / 66
页数:5
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