Nonomuraea guangzhouensis sp. nov., and Nonomuraea harbinensis sp. nov., two novel actinomycetes isolated from soil

Two novel actinomycetes, designated strains NEAU-ZJ3T and NEAU-yn31T, were isolated from soils in China and their taxonomic positions determined by a polyphasic approach. Key morphological and chemotaxonomic properties of the two strains were consistent with the description of the genus Nonomuraea. The G+C contents of strains NEAU-ZJ3T and NEAU-yn31T were determined to be 67.4 and 68.0 %, respectively. Phylogenetic analysis using 16S rRNA gene sequences indicated that the two strains should be classified in the genus Nonomuraea. The 16S rRNA gene sequence of strain NEAU-ZJ3T showed it to be most closely related to Nonomuraea salmonea DSM 43678T (98.27 %), Nonomuraea endophytica YIM 65601T (98.23 %) and Nonomuraea candida HMC10T (98.08 %), and that of strain NEAU-yn31T to Nonomuraeaferruginea IFO 14094T (99.08 %) and N. candida HMC10T (98.01 %). Furthermore, a combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that the two strains can be distinguished from each other and their phylogenetically closest relatives. Thus, strains NEAU-ZJ3T and NEAU-yn31T represent two novel species of the genus Nonomuraea, for which the names Nonomuraea guangzhouensis sp. nov. and Nonomuraea harbinensis sp. nov. are proposed. The type strains are NEAU-ZJ3T (=CGMCC 4.7101T = DSM 45889T) and NEAU-yn31T (=CGMCC 4.7106T = DSM 45887T), respectively.