Osteogenic and angiogenic effects of mesenchymal stromal cells with co-transfected human Ang-1 gene and BMP2 gene

被引：0
|
作者：
Xudong Liu
Bingfang Zeng
Changqing Zhang
机构：
[1] Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University,Department of Orthopaedic Surgery
来源：
Biotechnology Letters | 2011年 / 33卷
关键词：
Angiopoietin-1; Bone morphogenetic protein 2; Co-transfection; Lentiviral vector;
D O I：
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中图分类号：
学科分类号：
摘要：
To increase the osteogenic and angiogenic effects of marrow-derived mesenchymal stromal cells (MSCs), we co-transfected (by means of lentivirus) the human angiopoietin-1 gene (hAng-1) and human bone morphogenetic protein 2 gene (hBMP2) into MSCs. Real-time PCR and ELISA showed that both genes were successfully co-expressed in the MSCs with expression sustained until the eighth week. The alkaline phosphatase activity of the MSCs was more significantly augmented by the co-transfection with both genes than by any single gene transfection. These results demonstrate that the combined gene therapy with hAng-1 and hBMP2 using lentivirally co-transfected MSCs is feasible.
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页码：1933 / 1938
页数：5
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