Screening and large-scale expression of membrane proteins in mammalian cells for structural studies

被引：0
|
作者：
April Goehring
Chia-Hsueh Lee
Kevin H Wang
Jennifer Carlisle Michel
Derek P Claxton
Isabelle Baconguis
Thorsten Althoff
Suzanne Fischer
K Christopher Garcia
Eric Gouaux
机构：
[1] Vollum Institute,Departments of Molecular and Cellular Physiology and Structural Biology
[2] Oregon Health and Science University,undefined
[3] Howard Hughes Medical Institute,undefined
[4] Chevy Chase,undefined
[5] Stanford University School of Medicine,undefined
[6] Present address: Department of Physiology,undefined
[7] David Geffen School of Medicine,undefined
[8] University of California Los Angeles,undefined
[9] Los Angeles,undefined
[10] California,undefined
[11] USA.,undefined
来源：
Nature Protocols | 2014年 / 9卷
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摘要：
It can be difficult to express large amounts of membrane proteins for structural analysis. Using pEG BacMam it is possible to screen potential candidate proteins as well as to scale up expression in mammalian cells.
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页码：2574 / 2585
页数：11
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