Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

被引:0
|
作者
Woo Suk Ahn
Si Jae Park
Sang Yup Lee
机构
[1] Korea Advanced Institute of Science and Technology,Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center
来源
Biotechnology Letters | 2001年 / 23卷
关键词
cell recycle fed-batch culture; poly(3-hydroxybutyrate); recombinant ; whey;
D O I
暂无
中图分类号
学科分类号
摘要
A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l−1, 168 g l−1 and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l−1 as a feeding solution, resulting in a high productivity of 4.6 g PHB l−1 h−1.
引用
收藏
页码:235 / 240
页数:5
相关论文
共 50 条
  • [1] Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli
    Ahn, WS
    Park, SJ
    Lee, SY
    [J]. BIOTECHNOLOGY LETTERS, 2001, 23 (03) : 235 - 240
  • [2] Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution
    Ahn, WS
    Park, SJ
    Lee, SY
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) : 3624 - 3627
  • [3] Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter
    Park, SJ
    Park, JP
    Lee, SY
    [J]. BIOTECHNOLOGY LETTERS, 2002, 24 (03) : 185 - 189
  • [4] Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter
    Park S.J.
    Park J.P.
    Lee S.Y.
    [J]. Biotechnology Letters, 2002, 24 (3) : 185 - 189
  • [5] Production of poly(3-hydroxybutyrate) by fed-batch culture of filamentation-suppressed recombinant Escherichia coli
    Wang, FL
    Lee, SY
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (12) : 4765 - 4769
  • [6] Poly(3-hydroxybutyrate) production from whey using recombinant Escherichia coli
    Lee, SY
    Middelberg, APJ
    Lee, YK
    [J]. BIOTECHNOLOGY LETTERS, 1997, 19 (10) : 1033 - 1035
  • [7] Poly(3-hydroxybutyrate) production from whey using recombinant Escherichia coli
    S.Y. Lee
    A.P.J. Middelberg
    Y.K. Lee
    [J]. Biotechnology Letters, 1997, 19 : 1033 - 1035
  • [8] PRODUCTION OF POLY-BETA-HYDROXYBUTYRATE BY FED-BATCH CULTURE OF RECOMBINANT ESCHERICHIA-COLI
    KIM, BS
    LEE, SY
    CHANG, HN
    [J]. BIOTECHNOLOGY LETTERS, 1992, 14 (09) : 811 - 816
  • [9] Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture
    Quillaguaman, Jorge
    Doan-Van, Thuoc
    Guzman, Hector
    Guzman, Daniel
    Martin, Javier
    Everest, Akaraonye
    Hatti-Kaul, Rajni
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 78 (02) : 227 - 232
  • [10] Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture
    Jorge Quillaguamán
    Thuoc Doan-Van
    Héctor Guzmán
    Daniel Guzmán
    Javier Martín
    Akaraonye Everest
    Rajni Hatti-Kaul
    [J]. Applied Microbiology and Biotechnology, 2008, 78 : 227 - 232