Inhalation toxicity of indoor air pollutants in Drosophila melanogaster using integrated transcriptomics and computational behavior analyses

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作者
Hyun-Jeong Eom
Yuedan Liu
Gyu-Suk Kwak
Muyoung Heo
Kyung Seuk Song
Yun Doo Chung
Tae-Soo Chon
Jinhee Choi
机构
[1] School of Environmental Engineering,Department of Biological Sciences
[2] University of Seoul,Department of Physics
[3] The Key Laboratory of Water and Air Pollution Control of Guangdong Province,Department of Life Science
[4] South China Institute of Environmental Sciences,undefined
[5] the Ministry of Environment Protection of PRC,undefined
[6] Pusan National University,undefined
[7] Busandaehak-ro 63 beon-gil,undefined
[8] Geumjeoung-gu,undefined
[9] Busan 46241,undefined
[10] Korea,undefined
[11] Pusan National University,undefined
[12] Toxicity Evaluation Center,undefined
[13] Korea Conformity Laboratories (KCL),undefined
[14] 8,undefined
[15] University of Seoul,undefined
[16] Present address: Center for Nanosafety Metrology,undefined
[17] Korea Research Institute of Standards and Science (KRISS),undefined
[18] 267 Gajeong-Ro,undefined
[19] Yuseong-Gu,undefined
[20] Daejeon 34113,undefined
[21] Republic of Korea.,undefined
[22] Present address: Ecology and Future Research Association,undefined
[23] 21 Dusilro 45 beon-gil,undefined
[24] Geumjeong-gu,undefined
[25] Busan 46228,undefined
[26] Korea.,undefined
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摘要
We conducted an inhalation toxicity test on the alternative animal model, Drosophila melanogaster, to investigate potential hazards of indoor air pollution. The inhalation toxicity of toluene and formaldehyde was investigated using comprehensive transcriptomics and computational behavior analyses. The ingenuity pathway analysis (IPA) based on microarray data suggests the involvement of pathways related to immune response, stress response, and metabolism in formaldehyde and toluene exposure based on hub molecules. We conducted a toxicity test using mutants of the representative genes in these pathways to explore the toxicological consequences of alterations of these pathways. Furthermore, extensive computational behavior analysis showed that exposure to either toluene or formaldehyde reduced most of the behavioral parameters of both wild-type and mutants. Interestingly, behavioral alteration caused by toluene or formaldehyde exposure was most severe in the p38b mutant, suggesting that the defects in the p38 pathway underlie behavioral alteration. Overall, the results indicate that exposure to toluene and formaldehyde via inhalation causes severe toxicity in Drosophila, by inducing significant alterations in gene expression and behavior, suggesting that Drosophila can be used as a potential alternative model in inhalation toxicity screening.
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  • [1] Inhalation toxicity of indoor air pollutants in Drosophila melanogaster using integrated transcriptomics and computational behavior analyses
    Eom, Hyun-Jeong
    Liu, Yuedan
    Kwak, Gyu-Suk
    Heo, Muyoung
    Song, Kyung Seuk
    Chung, Yun Doo
    Chon, Tae-Soo
    Choi, Jinhee
    [J]. SCIENTIFIC REPORTS, 2017, 7