Rapid Quantitative HPTLC Analysis, on One Plate, of Emodin, Resveratrol, and Polydatin in the Chinese Herb Polygonum cuspidatum

被引:0
|
作者
Rui-zhi Zhao
Shaojun Liu
Li-lin Zhou
机构
[1] Guangdong Provincial Hospital of Traditional Chinese Medicine,The Pharmaceutical Laboratory
[2] Guangzhou University of Traditional Chinese Medicine,Bone Laboratory of First Affiliated Clinical College
[3] Guangzhou University of Traditional Chinese Medicine,Department of Pharmacy
来源
Chromatographia | 2005年 / 61卷
关键词
Thin-layer chromatography; Resveratrol; Emodin; Polydatin;
D O I
暂无
中图分类号
学科分类号
摘要
The rhizome of Polygonum cuspidatum is an important Chinese medicine used against infectious hepatitis, leucorrhagia, pruritus vulvae of the dampness-heat type, burns, snake bite, carbunculosis, amenorrhea, dysmenorrhea, trauma with blood stasis, and rheumatism, etc. Emodin, resveratrol, and polydatin are main active components of the rhizome. We report a simple densitometric HPTLC method for quantification of these compounds. The method was validated for precision, repeatability, and accuracy. The method was found to be precise, with RSD of 0.23, 0.25, and 0.32 (interday) and 0.45, 0.57, and 0.48 (intraday) for different concentrations of emodin, resveratrol, and polydatin, respectively. Instrument precision was 0.25, 0.23, and 0.34 (%CV) for emodin, resveratrol, and polydatin, respectively. The accuracy of the method was checked by measuring the recovery of the three compounds at three different levels; the average recoveries were 102.56%, 100.21%, and 100.27%, respectively. The amounts of emodin, resveratrol and polydatin in Polygonum cuspidatum, as estimated by the proposed method, were 4.96 mg g−1, 1.81 mg g−1, and 13.02 mg g−1. The HPTLC method proposed for estimation of emodin, resveratrol and polydatin was found to be simple, precise, specific, sensitive, and accurate and can be used for quality control of Polygonum cuspidatum.
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页码:311 / 314
页数:3
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