Characterization of the quantitatively major collagen in the mantle of oyster Crassostrea gigas

被引：0
|
作者：
Shoshi Mizuta
Tomoyuki Miyagi
Reiji Yoshinaka
机构：
[1] Fukui Prefectural University,Department of Marine Bioscience, Faculty of Biotechnology
来源：
Fisheries Science | 2005年 / 71卷
关键词：
bivalve; collagen; mantle; mollusc; muscle; oyster;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A quantitatively major collagen was isolated from the pepsin-solubilized collagen preparation of the mantle by differential salt precipitation and phosphocellulose column chromatography, and its constituent α components (α1 and α2) were purified by phosphocellulose column chromatography. The subunits were demonstrated to be genetically distinct from each other by peptide mapping and amino acid analysis. The amino acid composition calculated from those of the α1 and α2 components in 2∶1 ratio coincided well with that of the major collagen from the mantle. These results suggest that the major collagen in the mantle of the oyster may have a heterotrimer structure (α1)2 α2.
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页码：229 / 235
页数：6
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