IL-1 activates two phospholipid signaling pathways in intestinal epithelial cells

被引：0
|
作者：
F. R. Homaidan
I. Chakroun
G. S. Dbaibo
W. El-Assaad
M. E. El-Sabban
机构：
[1] American University of Beirut,
[2] Department of Physiology,undefined
[3] 850 Third Ave,undefined
[4] 18th floor,undefined
[5] AUB/NYC,undefined
[6] New York,undefined
[7] NY 10022,undefined
[8] Fax: +961 1 744 464,undefined
[9] e-mail: fh01@aub.edu.lb ,undefined
[10] Department of Pediatrics,undefined
[11] Faculty of Medicine,undefined
[12] American University of Beirut,undefined
[13] Beirut,undefined
[14] Lebanon ,undefined
[15] American University of Beirut,undefined
[16] Department of Human Morphology,undefined
[17] 850 Third Ave,undefined
[18] 18th floor,undefined
[19] AUB/NYC,undefined
[20] New York,undefined
[21] NY 10022,undefined
[22] Fax: +961 1 744 464,undefined
[23] e-mail: me00@aub.edu.lb ,undefined
来源：
Inflammation Research | 2001年 / 50卷
关键词：
Key words: Cyclooxygenase - Ceramide - IL-1 - Sphingomyelin - PLAP - PLA2 - Inflammation;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Objective and Design: the aim of the study was to decipher the molecular signals involved in IL-1's action on intestinal epithelial cells (IEC).¶Materials and Methods: Mode-K cells, used as a model of IEC, were treated with IL-1, and PLA2 activity and PGE2, ceramide, and cyclooxygenase-2 (COX-2) levels were measured using enzyme-immuno-assay kit, EIA, thin-layer chromatography and western blotting assays respectively.¶Results: IL-1 caused a concentration- and time-dependent increase in PLA2 activity (3-fold increase), in ceramide levels (peak increase = 10.5 ± 0.9 pmol/nmol phosphate), and in COX-2 and PGE2 levels. PGE2 increase was biphasic with an early peak at 10 min (around 5 ng/mg protein) due to increased PLA2 activity. The later peak (13.1 ± 1.9 ng/mg protein) at 4 h was due to COX-2 induction.¶Conclusion: In conclusion, these findings demonstrate that IL-1 regulates IEC function through two pathways, the PLA2 and the sphingomyelin pathways, both of which are capable of modulating the inflammatory process.
引用
收藏
页码：375 / 381
页数：6
相关论文
共 50 条
  • [1] IL-1 activates two phospholipid signaling pathways in intestinal epithelial cells
    Homaidan, FR
    Chakroun, I
    Dbaibo, GS
    El-Assaad, W
    El-Sabban, ME
    [J]. INFLAMMATION RESEARCH, 2001, 50 (07) : 375 - 381
  • [2] Activation of the sphingomyelin pathway in intestinal epithelial cells by IL-1
    Homaidan, FR
    El-Assaad, W
    Chakroun, I
    Dbaibo, G
    El-Sabban, ME
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 1998, 112 (01): : 46A - 47A
  • [3] Rapid expression of IL-1β by intestinal epithelial cells in vitro
    Waterhouse, CCM
    Stadnyk, AW
    [J]. CELLULAR IMMUNOLOGY, 1999, 193 (01) : 1 - 8
  • [4] Alternaria-Induced Releases Of IL-1 Alpha and IL-1 Beta From Airway Epithelial Cells Through Syk and PKC Signaling Pathways
    Sun, Yonghan
    Kita, Hirohito
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2014, 133 (02) : AB245 - AB245
  • [5] IL-1 beta promotes the development of intestinal epithelial stem cells
    Shi, Jishu
    Wang, Lei
    Liu, Ziyan
    Li, Yijing
    Pappan, Loretta
    [J]. CANCER RESEARCH, 2012, 72
  • [6] Interleukin-1β (IL-1β) induces a crosstalk between cAMP and ceramide signaling pathways in thyroid epithelial cells
    Schneider, C
    Rath, GM
    Delorme, N
    El Btaouri, H
    Hornebeck, W
    Martiny, L
    [J]. BIOCHIMIE, 2005, 87 (12) : 1121 - 1126
  • [7] Intracellular signaling pathways involved in IL-1β-induced ICAM-1 expression in human lung epithelial cells
    Lin, FS
    Yang, CM
    [J]. FASEB JOURNAL, 2004, 18 (05): : A946 - A946
  • [8] Il-1β induction of NF-κB activation in human intestinal epithelial cells is independent of oxyradical signaling
    Parikh, AA
    Moon, MR
    Pritts, TA
    Fischer, JE
    Szabó, C
    Hasselgren, PO
    Salzman, AL
    [J]. SHOCK, 2000, 13 (01): : 8 - 13
  • [9] Differences in signaling pathways by IL-1β and IL-18
    Lee, JK
    Kim, SH
    Lewis, EC
    Azam, T
    Reznikov, LL
    Dinarello, CA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (23) : 8815 - 8820
  • [10] IL-1β and the Intestinal Epithelial Tight Junction Barrier
    Kaminsky, Lauren W.
    Al-Sadi, Rana
    Ma, Thomas Y.
    [J]. FRONTIERS IN IMMUNOLOGY, 2021, 12
12345