Analysis of DNA sequencing systems based on capillary electrophoresis

被引：0
|
作者：
A. Stepukhovich
A. Tsupryk
O. Kosobokova
D. N. Gavrilov
B. Gorbovitski
G. Gudkov
G. Tyshko
M. Tcherevishnik
V. Gorfinkel
机构：
[1] State University of New York,Department of Electrical and Computer Engineering
来源：
Technical Physics | 2008年 / 53卷
关键词：
82.45.Wx;
D O I：
暂无
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学科分类号：
摘要：
A method for characterization and optimization of DNA sequencing systems based on capillary electrophoresis is proposed. A model is developed that relates the read length of a sequencing run and the parameters of DNA sequencing systems such as the properties of the separation medium, capillary length, injection time, concentration of the injected DNA sample, detection sensitivity, system noise, and the illumination power. The optimization of DNA sequencing systems will allow determination of optimum regimes for preparation, injection, and separation of DNA samples needed for achieving a read length required. The method proposed is applied to the characterization of capillary-electrophoretic DNA sequencing systems based on single-photon detection.
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