Features of the local immobilization of NADPH-cytochrome P450 reductase and cytochrome b5 on a solid surface

被引:0
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作者
I. V. Paribok
G. K. Zhavnerko
V. E. Agabekov
A. V. Yantsevich
S. A. Usanov
机构
[1] National Academy of Sciences of Belarus,Institute of Chemistry of New Materials
[2] National Academy of Sciences of Belarus,Institute of Bioorganic Chemistry
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PDMS; General Chemistry; Atomic Force Microscopy Image; Chimeric Protein; Step Height;
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摘要
Using the methods of microcontact printing and atomic force microscopy, we studied a possibility of specific “protein-protein” interactions in the processes of local immobilization of cytochrome b5 and NADPH-cytochrome P450 reductase on the surface of hydrophilic silicone. It is shown that the formation of protein complexes is possible only at a certain orientation of the molecules defined by the sequence of adsorption of biopolymers.
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页码:1060 / 1063
页数:3
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