PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings

被引：0
|
作者：
G. Mohanty
J. Mohanty
S. K. Garnayak
S. K. Dutta
机构：
[1] North Orissa University,Department of Biotechnology
[2] Central Institute of Freshwater Aquaculture,Department of Zoology
[3] North Orissa University,undefined
来源：
Veterinary Research Communications | 2009年 / 33卷
关键词：
DNA damage, Furadan, ; , RAPD; PCR;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The present study involves the use of RAPD-PCR to evaluate the genotoxic effects of furadan in the DNA of Labeo rohita (rohu) fingerlings. Rohu fingerlings were exposed to 0.02 ppm of furadan for a total period of 96 h and samplings were done at 24, 48, 72 and 96 h. RAPD - PCR were carried out with the blood and liver DNA samples of both control and treated groups at each of the four sampling hours. A total of six selected RAPD primers were used for PCR amplification. Template stability has been taken as the measure of DNA damage caused by pesticide. The results obtained showed no significant difference in the template stability in the blood DNA of furadan treated groups at any of the four sampling hours; however, the liver DNA were able to show significant difference at 48 and 96 hours of treatment.
引用
收藏
页码：771 / 780
页数：9
相关论文
共 50 条
  • [1] PCR based detection of furadan genotoxicity effects in rohu (Labeo rohita) fingerlings
    Mohanty, G.
    Mohanty, J.
    Garnayak, S. K.
    Dutta, S. K.
    VETERINARY RESEARCH COMMUNICATIONS, 2009, 33 (07) : 771 - 780
  • [2] PCR based Detection of Phorate Genotoxicity Effects in Rohu (Labeo rohita) Fingerlings
    Mohanty, G.
    Mohanty, J.
    Garnayak, S. K.
    Dutta, S. K.
    RESEARCH JOURNAL OF BIOTECHNOLOGY, 2009, 4 (02): : 23 - 30
  • [3] Dietary magnesium requirement for fingerlings of Rohu (Labeo rohita)
    Musharraf, Mohammad
    Khan, Mukhtar A.
    AQUACULTURE, 2018, 496 : 96 - 104
  • [4] EFFECTS OF TEMPERATURE ON EGGS, FRY, AND FINGERLINGS OF ROHU (LABEO-ROHITA) EXPOSED TO UREA
    SARKAR, SK
    PROGRESSIVE FISH-CULTURIST, 1991, 53 (04): : 242 - 245
  • [5] Effects of thermostable bacterial α-amylase on growth and feed utilization in rohu, Labeo rohita (hamilton), fingerlings
    Ghosh, K
    Chakraborty, K
    Sen, SK
    Ray, AK
    ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, 2001, 53 (3-4): : 101 - 109
  • [6] Utilization of copra meal in the formulation of compound diets for rohu, Labeo rohita, fingerlings
    Mukhopadhyay, N
    Ray, AK
    JOURNAL OF APPLIED ICHTHYOLOGY, 1999, 15 (03) : 127 - 131
  • [7] Pathophysiological Changes in Rohu (Labeo rohita, Hamilton) Fingerlings Following Arsenic Exposure
    Mohanty, Bimal Prasanna
    Banerjee, Sudeshna
    Sadhukhan, Papia
    Chowdhury, Atalanta Narayan
    Golder, Debashree
    Bhattacharjee, Soma
    Bhowmick, Sanjay
    Manna, Sanjib Kumar
    Samanta, Srikanta
    NATIONAL ACADEMY SCIENCE LETTERS-INDIA, 2015, 38 (04): : 315 - 319
  • [8] Pathophysiological Changes in Rohu (Labeo rohita, Hamilton) Fingerlings Following Arsenic Exposure
    Bimal Prasanna Mohanty
    Sudeshna Banerjee
    Papia Sadhukhan
    Atalanta Narayan Chowdhury
    Debashree Golder
    Soma Bhattacharjee
    Sanjay Bhowmick
    Sanjib Kumar Manna
    Srikanta Samanta
    National Academy Science Letters, 2015, 38 : 315 - 319
  • [9] Dietary ascorbic acid requirements of fingerlings of genetically improved rohu (Labeo rohita Ham)
    Sahoo, Minakshi
    Mukhopadhyay, P. K.
    ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH, 2008, 60 (02): : 100 - 106
  • [10] Modulation of cytokine expression by dietary levan in the pathogen aggravated rohu, Labeo rohita fingerlings
    Gupta, Sanjay K.
    Sarkar, B.
    Bhattacharjee, S.
    Kumar, Neeraj
    Naskar, S.
    Uppuluri, Kiran Babu
    AQUACULTURE, 2018, 495 : 496 - 505
12345