Adventitious bud induction in Eucalyptus globulus Labill

被引:0
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作者
Greg Nugent
Stephen F. Chandler
Phil Whiteman
Trevor W. Stevenson
机构
[1] Royal Melbourne Institute of Technology,Department of Applied Biology and Biotechnology
[2] Florigene Pty. Ltd,Department of Biology
[3] Australian Paper Plantations Pty. Ltd,undefined
[4] National University of Ireland,undefined
关键词
mature embryo; organogenesis; thidiazuron;
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学科分类号
摘要
Adventitious buds and shoots of Eucalyptus globulus Labill. (Tasmanian Bluegum) have been regenerated from cotyledons and hypocotyls from mature embryos and seedlings. Adventitious buds, were induced at high frequency with 0.05 μM thidiazuron in combination with 0.2 μM 2,4-dichlorophenoxyacetic acid or 5 μM α-naphthaleneacetic acid. Culture of explants in the dark inhibited bud induction, but up to 86% of cotyledons, longitudinally split just prior to culture, produced adventitious buds, in the light. Development of buds into shoots occurred only at low frequency, after transfer to media containing N6-benzylaminopurine.
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页码:388 / 391
页数:3
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