Enantioseparation Using Chitosan Tris(3-chlorophenylcarbamate) as a Chiral Stationary Phase for HPLC

A novel chiral stationary phase (CSP) consisting of a silica-based chitosan tris(3-chlorophenylcarbamate) derivative was used for the enantioseparation of nine racemic compounds in the normal phase mode by high-performance liquid chromatography (LC). The influence of the type and percentage of modifier in the mobile phase on the optimization of the resolution was investigated. The resolution of epoxiconazole using this system was compared with those achieved using commercially available Chiralcel® OD-H and Chiralpak® AD-H columns. Among the tested racemates, seven racemic compounds were separated most quickly on the CSP of chitosan tris(3-chlorophenylcarbamate). Baseline or near-baseline separation was achieved for benzoin, penconazole, hexaconazole, and epoxiconazole, while the others were partially separated. The enantioseparation results for epoxiconazole were not inferior to those of commercially available Chiralcel® OD-H and Chiralpak® AD-H columns.