Expression of Bacillus sp. β-xylosidase gene (xylB) in Saccharomyces cerevisiae

被引:0
|
作者
Jong-Hyun Kim
Byung-Woo Kim
Ki-Hong Yoon
Soo-Wan Nam
机构
[1] Dong-Eui University,Department of Microbiology
[2] Woosong University,School of Food Biotechnology
来源
Biotechnology Letters | 2000年 / 22卷
关键词
β-xylosidase; exoinulinase signal sequence;
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摘要
β-Xylosidase gene (xylB) from Bacillus sp. was amplified and inserted between GAL10 promoter and GAL7 terminator. For the secretory production of xylB in Saccharomyces cerevisiae, in-frame fusion of the exoinulinase signal sequence (INU1s) of Kluyveromyces marxianus to the upstream of xylB was conducted. When a transformant of S. cerevisiae harboring the resulting plasmid was grown on galactose-containing medium, most of β-xylosidase activity was localized in the periplasmic space of yeast and a maximum total activity reached about 2.9 unit ml−1 at 42 h cultivation. The recombinant β-xylosidase was produced as an active dimer form.
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页码:1025 / 1029
页数:4
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