Complete mitogenome sequencing of Andaman buffalo: an endangered germplasm of Andaman and Nicobar Islands, India

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作者
Arun Kumar De
Perumal Ponraj
Dhruba Malakar
Ramachandran Muthiyan
A. Kundu
Debasis Bhattacharya
机构
[1] ICAR-Central Island Agricultural Research Institute,Animal Science Division
[2] ICAR-Central Island Agricultural Research Institute,Bioinformatics Centre
[3] National Dairy Research Institute,Animal Biotechnology Centre
[4] GCC Biotech India Pvt. Ltd.,undefined
来源
Journal of Genetics | 2019年 / 98卷
关键词
Andaman buffalo; mitogenome; illumina sequencing; Andaman and Nicobar Islands;
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摘要
Andaman buffalo is an indigenous buffalo of Andaman and Nicobar Islands, India. Over the last decade, it has witnessed a rapid decline in population, necessitating its immediate characterization and conservation. The present study reports the complete mitogenome profile of Andaman buffalo which is 16,359 bp in length and comprised of 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNAs and two ribosomal RNAs. In addition, one A+T\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\hbox {A}\,+\,\hbox {T}$$\end{document} rich region (D-loop) was also present. A biasness towards A and T base was observed in all the genes. All the PCGs except ND6 were present on heavy strand. Start codons for all the 13 PCGs were ATN codon and abbreviated/truncated stop codons were observed in ND1, ND2, COX3, ND3 and ND4. The phylogenetic analysis revealed that the Andaman buffalo is closely related to buffalo from India and China. The results from this study will help in sketching the conservation plan of the threatened breed.
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