Isolation of polymorphic microsatellite markers from amphioxus (Branchiostoma belcheri) and a test of cross-species amplification

被引：0

作者：

Yun-Guo Liu

Ling-Xiao Liu

Zhao-Xia Li

Fang-Zhen Li

Chun-Ying Liu

机构：

[1] Ocean University of China,

[2] Inspection & Quarantine Technology Center,undefined

[3] Shandong Entry-Exit Inspection & Quarantine Bureau,undefined

[4] Linyi Academy of Agricultural Sciences,undefined

[5] Qingdao Agricultural University,undefined

来源：

Conservation Genetics Resources | 2009年 / 1卷

关键词：

Amphioxus; Microsatellite; RAPD; Cross-species amplification;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Eleven pairs of simple sequence repeat markers (SSR) were developed from RAPD product in amphioxus, Branchiostoma belcheri. Fifty microsatellites were selected for designing microsatellite primers, of which 11 gave working primer pairs. They had between three and eleven alleles. Observed and expected heterozygosities varied from 0.65 to 0.90, and from 0.59 to 0.86, respectively. Five additional fish species of the vertebrates assessed for cross-species amplification revealed between two and four positive amplifications and between zero and two polymorphic loci per species.

引用

页码：257 / 259

页数：2

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