Effect of growth regulators on in vitro multiplication and rooting of shoot cultures in sugarcane

An experiment was carried out to study the effect of growth regulators on in vitro multiplication of shoot cultures and their rooting in sugarcane varieties CoS 99259 and CoSe 01235. About 1.5 cm long shoot tip expiants comprising apical meristems and 1-2 leaf primordia were carefully excised from the apical region, sterilized and inoculated on agar (8.0 g/1) gelled MS medium supplemented with various concentrations and combinations of BAP, kinetin and NAA. The results showed that MS medium supplemented with BAP, Kinetin and NAA (0.5 mg/1 each) was most suitable for producing optimum number of shoots in both the varieties. For rooting, the multiplying cultures were separated in smaller groups and transferred on half strength MS liquid medium containing 50g/1 sucrose and different concentrations of auxins (NAA and IBA). Maximum 94% shoot cultures developed vigorous root system on half strength MS liquid medium containing 50 g/1 sucrose and 5.0 mg/1 NAA. © 2009 Society for Sugar Research & Promotion.